MARC details
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| fixed length control field |
04192nam a22004215i 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20230718140420.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
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| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
210202t20142015pl fo d z eng d |
| 019 ## - |
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| -- |
(OCoLC)1013967206 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
9783110411331 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
DE-B1597 |
| Language of cataloging |
eng |
| Transcribing agency |
DE-B1597 |
| Description conventions |
rda |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Gerczei Fernandez, Timea, |
| Relator term |
author. |
| Relator code |
aut |
| -- |
http://id.loc.gov/vocabulary/relators/aut |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Biochemistry Laboratory Manual For Undergraduates : |
| Remainder of title |
An Inquiry-Based Approach / |
| Statement of responsibility, etc. |
Timea Gerczei Fernandez, Scott Pattison. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
Warsaw ; |
| -- |
Berlin : |
| Name of producer, publisher, distributor, manufacturer |
De Gruyter Open Poland, |
| Date of production, publication, distribution, manufacture, or copyright notice |
[2014] |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Date of production, publication, distribution, manufacture, or copyright notice |
©2015 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
1 online resource (186 p.) |
| 336 ## - CONTENT TYPE |
|---|
| Content type term |
text |
| Content type code |
txt |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
|---|
| Media type term |
computer |
| Media type code |
c |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
|---|
| Carrier type term |
online resource |
| Carrier type code |
cr |
| Source |
rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS |
|---|
| File type |
text file |
| Encoding format |
PDF |
| Source |
rda |
| 505 00 - FORMATTED CONTENTS NOTE |
|---|
| Title |
Frontmatter -- |
| -- |
Preface -- |
| -- |
Contents -- |
| -- |
1 Introducing the Bacterial Antibiotic Sensor Mini Project -- |
| -- |
2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- |
| -- |
3 Designing Primers for Site-Directed Mutagenesis -- |
| -- |
4 Performing Site-Directed Mutagenesis -- |
| -- |
5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- |
| -- |
6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- |
| -- |
7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- |
| -- |
8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- |
| -- |
9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- |
| -- |
10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- |
| -- |
11 Understanding the Importance of Buffers in Biological Systems -- |
| -- |
12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- |
| -- |
13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- |
| -- |
14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- |
| -- |
List of Figures |
| 506 0# - RESTRICTIONS ON ACCESS NOTE |
|---|
| Terms governing access |
Open Access |
| Uniform Resource Identifier |
<a href="https://purl.org/coar/access_right/c_abf2">https://purl.org/coar/access_right/c_abf2</a> |
| Standardized terminology for access restriction |
unrestricted online access |
| Source of term |
star |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Biochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic. |
| 538 ## - SYSTEM DETAILS NOTE |
|---|
| System details note |
Mode of access: Internet via World Wide Web. |
| 540 ## - TERMS GOVERNING USE AND REPRODUCTION NOTE |
|---|
| Terms governing use and reproduction |
This eBook is made available Open Access. Unless otherwise specified individually in the content, the work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND) license: |
| Uniform Resource Identifier |
<a href="https://creativecommons.org/licenses/by-nc-nd/3.0 ">https://creativecommons.org/licenses/by-nc-nd/3.0 </a> |
| -- |
<a href="https://www.degruyter.com/dg/page/open-access-policy">https://www.degruyter.com/dg/page/open-access-policy</a> |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
In English. |
| 588 0# - SOURCE OF DESCRIPTION NOTE |
|---|
| Source of description note |
Description based on online resource; title from PDF title page (publisher's Web site, viewed 02. Feb 2021) |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
SCIENCE / Life Sciences / Biochemistry. |
| Source of heading or term |
bisacsh |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Pattison, Scott, |
| Relator term |
author. |
| Relator code |
aut |
| -- |
http://id.loc.gov/vocabulary/relators/aut |
| 776 0# - ADDITIONAL PHYSICAL FORM ENTRY |
|---|
| Qualifying information |
EPUB |
| International Standard Book Number |
9783110426250 |
| 776 0# - ADDITIONAL PHYSICAL FORM ENTRY |
|---|
| Qualifying information |
print |
| International Standard Book Number |
9783110411324 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://doi.org/10.2478/9783110411331">https://doi.org/10.2478/9783110411331</a> |
| Public note |
Open Access |
| -- |
0 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://www.degruyter.com/isbn/9783110411331">https://www.degruyter.com/isbn/9783110411331</a> |
| Public note |
Open Access |
| -- |
0 |
| 856 42 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Materials specified |
Cover |
| Uniform Resource Identifier |
<a href="https://www.degruyter.com/cover/covers/9783110411331.jpg">https://www.degruyter.com/cover/covers/9783110411331.jpg</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
E-Book |
