Cytotoxic test of helianthus anuus linn. (sunflower) seed

Thesis Bachelor of Science in Biology University of Rizal System-Morong 2019

EXECUTIVE SUMMARY: The study aimed to find the cytotoxic activity of sunflower seed extract. It was conducted from September 2018 to January 2019, at university of rizal system, morong campus, morong, rizal during the school year 2018-2019. Experimental research design was employed in the study. The extraction sunflower seed was done at the college of science building, university of rizal system, morong campus. The cytotoxic assay was conducted at the biological research and service laboratory of the natural science research institute, university of the Philippines- diliman, quezon city. To determine the cytotoxic test of sunflower seed extract, 3-(4,5-dimethiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (mtt) assay was used. It is a test that is wisely used to identify cytotoxic potential of plant. The mtt assay is a reliable and convenient method for identifying toxicity which can measure cell proliferation, viability and cytotoxicity. The test subject used is sunflower seed since it is use by many and genetically known for its uses. In determining the rate of cytotoxic test in terms of percent dead cells of sunflower seed extract, roswell park memorial institute (rpmi's) media), a known cell growth promoter, and triton x-100's, a known cytotoxic agent were used. The cytotoxic activity of sunflower seed extract were determined by calculating the percentage survival of the cell. The result of the mtt assay shows that, sunflower seed extract that the percent dead cells is close to triton x-100's percent dead cells and distal to the rpmi's percent dead cells. Based from the findings of the study it its concluded that sunflower seed is cytotoxic with consideration to the amount of sunflower seed consumed.