Characterization, identification, and antibiotic activity of isolated Michael E. Casono... [et. al]

Thesis Bachelor of Science in Biology University of Rizal System-Morong. 2018

EXECUTIVE SUMMARY: The study aimed to characterize further the collected Actinomycetes, which were isolated by previous researchers, found in soil from Mount Sembrano, Malaya, Pililla, Rizal and to determine the identification of the isolates that can produce antibiotics. Also, the study was performed to classify the Actinomycetes isolated samples that have antibiotic activity. The study was conducted from November to September 2018 at the University of Rizal System SY. 2017-2018. The study utilized the descriptive-experimental design where the characterization of Actinomycetes was determined and analyzed. The procedures used during the study were gathering of data, preparation of selective media, re-streaking of pure Actinomycetes, re-slanting, examination of morphological examination of isolated Actinomycetes, preparation of nutrient agar, test for antibiotic activity, examination of inhibition of indicator organisms, staining for identification of the isolated Actinomycetes with active and very active antibiotic performance, and lastly gathering, analysis and interpretation of data. The result of the study revealed that the morphology of forty-three (43) isolates were filamentous, curled, and entire. Moreover, the result from antibiotic potential test revealed that Actinomycetes had active antibiotic activity. Two (2) out of forty-two (42) isolates had active antibiotic performance against Streptococcus pneumoniae; both three (3) active and very active out of forty-two (42) isolates against Salmonella typhimurium; and six (6) out of forty-two (42) were active against Pseudomonas aeruginosa. By the end of the experiment, the researchers had classified four genera under order Actinomycetales. The isolates with active and very active antibiotic performance against selected pathogens such as Salmonella typhimurium, Streptococcus pneumoniae, and Pseudomonas aeruginosa were identified. Five (5) from genus Actinoplanes, three (3) from genus Streptomyces, one (1) from genus Pilimelia, and one (1) from Thermomonospora were identified after morphological inspection and Gram-staining test. It was therefore concluded that based from the findings of the study, the isolated Actinomycetes in soil from Mount Sembrano can be identified in terms of genus rank, characterized, and have antibiotic activity against Salmonella typhimurium, Streptococcus pneumonia, Pseudomonas aeruginosa.