Antiangiogenic activity of bulak-manok (ageratum conyzoides l.) Arthur Allan S. Leones... [et. al]

Thesis (Bachelor of Science in Biology) -- University of Rizal System-Morong.

EXECUTIVE SUMMARY: The study aimed to determine the activity of Bulak-Manok Leaves extract as antiangiogenic agent. The leaves extract of Bulak-Manok was used in the study and compared its effect with commercially prepared antiangiogenic (retonic Acid) and proangiogenic (95& Ethanol) substances. The study was conducted at the University of Rizal System Morong during the school year 2016-2017. The study used thirty (30) pieces of one day old fertile duck eggs. Leaves of Bulak-Manok were immersed to 95% ethanol for two dyas to produce the crude ethanolic extract. The processes used in the study involved the gathering of materials, incubation of the one day old fertile duck eggs and following the Chorioallantoic Membrane (CAM) Assay. To determine the antiangiogenic activity of Bulak-Manok leaves extract, Chorioallantoic Membrane (CAM) Assay was utilized. It is a favored system for the study of tumor angiogenesis and metastasis. Also, this test is quick, technically simple, and inexpensive. The test organism used is one day old fertile duck (Anas platyrhynchos domesticus L.) eggs and the subject of the study which is Bulak-Manok (Ageratum conyziodes L.) leaves. The study utilized descriptive method of research in determining the antiangiogenic acticity of Bulak-Manok leaves extract. The CAMs were exposed to filter papers treated with Pure Concentration of Bulak-Manok Leaves Extract, retonic acid served as inhibitor and 95% Ethanol served as promoter respectively. The antiangiogenic activity of Bulak-Manok leaves extract was evaluated according to the Chorioallantoic Membrane (CAM) Assay. This was determined on the 12th day of incubation. Mean and standard deviation were used to determine the rate of the number of blood vessel branch points on duck CAMs exposed to filter papers treated with Pure Concentration of Bulak-Manok Leaves Extract, Retinoic Acid served as inhibitor and 95% Ethanol (Promoter). To determine the CAMs vascularity, the percentage of CAMs vascularity between the pure concentration of Bulak-Manok Leaves Extract, Retinoic Acid (Inhibitor), and 95% Ethanol (Promoter). To determine the CAMs vascularity, the percentage of CAMs vascularity between the Pure Concentration of Bulak-Manok leaves extract and inhibitor and between the said extract and the promoter were calculated. One-way Analysis of Variance was utilized to determine the significant difference on the rate of vascularity of CAMs exposed to filter papers treated with different treatments. The results revealed that, pure concentration of Bulak-Manok leaves extract has strong inhibitory activity against the blood vessel branch points of Duck CAMs. Considering the low number of blood vessel branch points present in Duck CAMs, the study concluded that pure concentration of Bulak-Manok leaves extract has antiangiogenic activity. Based on the conclusion, the study recommended that pure concentration of Bulak-Manok leaves extract may be used to inhibit the proliferation of blood vessels needed by the tumors to sustain its existence. Moreover, it suggested further studies on stability of Bulak-Manok leaves extract and phytochemical screening to determine the exact and specific components of the Bulak-Manok leaves extract which are responsible for its antiangiogenic activity.