Cytotoxic activity of brewed oryza sativa l. (rice) utilizing real-time Royette C. Cruz... [et. al]

Thesis (Bachelor of Science in Biology) -- University of Rizal System-Morong.

EXECUTIVE SUMMARY: The study aimed to determine the cytotoxic activity on the Brewed Rice Utilizing Real-Time Whole Blood Assay, the study also aimed to give awareness to people if brewed rice is safe for consumption as an alternative beverage. The study was conducted from June 2017 to September 2017, at University of Rizal System-Morong, Rizal SY: 2016-2017. Descriptive research design were employed in the study. The extraction of Brewed Rice were done at Laboratory Room #1, College of Science Building of University of Rizal System, Morong Campus. The cytotoxicity test was conducted at the National Science Research Institute at Miranda Hall, University of the Philippines Diliman Quezon, City, Metro Manila. To determine the cytotoxic activity of Brewed Rice Extract, trypan blue method was used, which is widely used to identify the cytotoxicity of foods, beverages as well as plant materials and their direct and indirect cytotoxicity. The study made use of human blood assay to determine the cytotoxicity effect of brewed ice. To determine if brewed rice is carcinogenic, it was compared with positive control (Roswell Park Memorial Institute (RPMI) medium) and negative control (Triton X-100). Number of target live and dead cells were considered as the parameter. Mean and standard deviation were used to determine the rate of effect of each treatment while One-Way Analysis of Variance was utilized to determine the significant difference in the effect of each treatment on cells. Result shows that Brewed Rice Extract has Cytotoxic effect after utilizing human whole-blood assay, since there is low number of live cells in the microcentrifuge tubes as compared to controls. Based on the findings of the study, it shows that brewed rice might impose carcinogenic effect on human health if used as an alternative beverage or coffee.