Application of molecular methods and Raman microscopy/spectroscopy in agricultural sciences and food technology / editors: Biljana Vucelić Radović, Dejan Lazić, Miomir Nikšić.

Includes bibliographical references.

Part I. PCR in agricultural sciences and food technology - Plant sciences - Two-step RT-qPCR analysis of expression of 7 drought-related genes in tomato (Lycopersicon esculentum Mill.) / Ivana Petrović - Application of molecular methods in weed science / Dragana Božić, Markola Saulić and Sava Vrbničanin - DNA extraction and application of SSR markers in genetic identification of grapevine cultivars / Zorica Ranković-Vasić and Dragan Nikolić - Microorganisms - Application of qPCR method for investigation of plant colonization by human pathogen bacteria / Kljujev Igor - The application of molecular methods in diagnostics of phytopathogenic viruses, fungi and fungus-like organisms / Ivana Stanković and Ana Vučurović - Real-time PCR detection of quarantine plant pathogenic bacteria in potato tubers and olive plants / Milan Ivanović, Nemanja Kuzmanović and Nevena Zlatković - Food biochemistry and technology - Application of PCR in food biochemistry / Milica Pavlićević and Biljana Vucelić-Radović - Fishery - Application of molecular methods in aquaculture and fishery / Zorka Dulić, Božidar Rašković, Saša Marić and Tone-Kari Knutsdatter Østbye - Part II. Raman microscopy/spectroscopy in agricultural science and food technology - Introduction to Raman microscopy/spectroscopy / Dejan Lazić - Working conditions in the laboratory for Raman microscopy / Steva Lević - Characterization of microorganisms using Raman microscopy / Danka Radić - Polysaccharide mushroom extracts : digging into the unknown / Jovana Vunduk - Application of Raman microscopy for dairy products analysis / Aleksandar Nedeljković - Raman microscopy in plant science, carotenoids detection in fruit material / Ilinka Pećinar - Materials characterization by Raman microscopy / Steva Lević - Polarized light microscopy / Dragana Rančić - Short instructions for Raman microscope Horiba Xplora / Dejan Lazić.

PDF (JSTOR, viewed August 12, 2019).

"This book has been prepared with the aim to present the application of these two state-of-the art technologies in agricultural sciences and food technology, and to explain the protocols for analyses of different plant, animal, microbiological and food samples as well as for different biotechnology procedures. Selected methods and protocols which are used in plant stress physiology, weed science, fruit breeding research, microbial ecology, plant virus and fungus diagnostics, phytobacteriology, fishery, food biochemistry, food materials and food technology are described. Special adaptation of certain protocols is required for application in each of these sciences, for every type of GMO organism, food technology raw material, and food technology product, as well as for every type of bacteria, virus, fungus or fungus-like organism, for each type of raw material in terms of plant host species, plant organs, year period and conditions in the laboratory. Application of molecular methods, primarily qPCR, and Raman microscopy/ spectroscopy in agricultural and food sciences provides substantial opportunity for increased production efficiency, food safety, better product quality and improvement of plant and animal health. This book is aimed for students, scientists and professionals working in the field of agriculture and food technology."

English.

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