Total phytochemical analysis of Mikania cordata burm.f. B.L. Rob (Bikas) for potential multiple uses and application / Roger S. Sabacajan and Ophrah Jae pages Subida

Descriptive Research Methodology

Thesis BS Biology University of Rizal System. Cardona 2018

The study aimed to evaluate the total phytochemical analysis of Mikania cordata Burm.f. B.L. Rob for multiple uses application. The researchers made use of the descriptive design to determine the phytochemicals present in the leaves, stem, and roots of M. cordata qualitatively and quantitatively. The actual study made use of the following procedures: collection, extraction, and laboratory testing of the leaves, stem and roots of M. cordata. The plant was collected in Cardona, Rizal. The analysis of phytochemical constituents of the collected leaves, stem and roots of M. cordata was done at the Adamson University Technology Research and Development Center (AUTRDC) located at San Marcelino St. Ermita Manila. To determine the phytochemical constituents present in the plant, qualitative analysis was used by the experts from AUTRDC. Lead acetate was used to determine flavonoids which showed that it was present in stem, roots and leaves. Ferric chloride test was used to determine tannin which showed that it was present in stem. Roots and leaves, Dragendorff's test was used to determine alkaloids which was absent in stem, roots and leaves. Keller - killani test was used to determine glycosides which showed to be present on leaves. Froth test was used to determine the saponin which showed that it was present in roots. To quantify the determined constituents, quantitative analysis was done. Spectrophotomeric method was used to quantify flavonoids and tannins. The results revealed that 171 mg/L of flavonoids and 245 mg/L were present in leaves. 144mg/L of flavonoids and 336mg/L of tannins were determined in roots. 131mg/L of flavonoids and 157mg/L of tannins were present in the stem. Solvent extraction and Gravimetric showed 18.02mg/L of saponins were present on roots. Through the said result, the researchers concluded that M. cordata could be seen as a good source of useful drug and the abundance of tannin and flavonoids confirmed the potential of the plant for medical and industrial uses and application.

Sabacajan, R. S. and Subida, O. J. (2018). Total phytochemical analysis of Mikania cordata burm.f. B.L. Rob (Bikas) for potential multiple uses and application. Cardona: University of Rizal System.