000			04192nam a22004215i 4500
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007			cr || ||||||||
008			210202t20142015pl fo d z eng d
019			_a(OCoLC)1013967206
020			_a9783110411331
040			_aDE-B1597 _beng _cDE-B1597 _erda
041	0		_aeng
100	1		_aGerczei Fernandez, Timea, _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	0	_aBiochemistry Laboratory Manual For Undergraduates : _bAn Inquiry-Based Approach / _cTimea Gerczei Fernandez, Scott Pattison.
264		1	_aWarsaw ; _aBerlin : _bDe Gruyter Open Poland, _c[2014]
264		4	_c©2015
300			_a1 online resource (186 p.)
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
505	0	0	_tFrontmatter -- _tPreface -- _tContents -- _t1 Introducing the Bacterial Antibiotic Sensor Mini Project -- _t2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- _t3 Designing Primers for Site-Directed Mutagenesis -- _t4 Performing Site-Directed Mutagenesis -- _t5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- _t6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- _t7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- _t8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- _t9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- _t10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- _t11 Understanding the Importance of Buffers in Biological Systems -- _t12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- _t13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- _t14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- _tList of Figures
506	0		_funrestricted online access _2star
520			_aBiochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
538			_aMode of access: Internet via World Wide Web.
540			_aThis eBook is made available Open Access. Unless otherwise specified individually in the content, the work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND) license: _uhttps://creativecommons.org/licenses/by-nc-nd/3.0 _uhttps://www.degruyter.com/dg/page/open-access-policy
546			_aIn English.
588	0		_aDescription based on online resource; title from PDF title page (publisher's Web site, viewed 02. Feb 2021)
650		7	_aSCIENCE / Life Sciences / Biochemistry. _2bisacsh
700	1		_aPattison, Scott, _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut
776	0		_cEPUB _z9783110426250
776	0		_cprint _z9783110411324
856	4	0	_uhttps://doi.org/10.2478/9783110411331 _zOpen Access _70
856	4	0	_uhttps://www.degruyter.com/isbn/9783110411331 _zOpen Access _70
856	4	2	_3Cover _uhttps://www.degruyter.com/cover/covers/9783110411331.jpg
942			_cE-BOOK
999			_c71334 _d71333