Undergraduate Thesis
Bachelor of Science in Agriculture major in Crop Science
University of Rizal system, Rodriguez, Rizal
2025

The purpose of this study was to determine the Utilization of Marigold (Tagetes erecta) Extract as a Botanical Insecticide for Aphids using Randomized Block Design with three treatments and replications totaling nine experimental units. Each experimental unit went through 10 trials contained 10 aphids each cup, the researchers applied Analysis of Variance (ANOVA) in order to determine the significant difference in the data.
The experiment was conducted at University of Rizal System, Rodriguez Campus, T. Reyes St., Amity Ville, San Jose, Rodriguez, Rizal. The study used nine hundred (900) aphids as an experimental subject. The experiment was conducted from October 23 to December 5, 2024, lasting a total of 43 days.
The study utilized marigold extract as a botanical insecticide to determine its effectiveness in terms of time and dosage every ten (10) aphids per trials in each replication. The objective of this study was to assess the effectiveness of botanical insecticide in terms of time and dosage. The experiment applied marigold extract with different concentrations. Treatment 1 used 100 ml distilled water as controlled group, in treatment 2 used 25 ml of marigold extract diluted to 100 ml of distilled water, in treatment 3 used 50 ml of marigold extract diluted to 100 ml of distilled water and record the duration of its effect.
This study showed that utilization of marigold extract as a botanical insecticide has a potential benefit in terms of time and dosage, it shows that treatment 3 with 50 ml marigold extract diluted to 100 ml of distilled water proved to be the most potent, resulting in substantial mortality. The findings showed that
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the extended the time of exposure and the higher the dosage of a botanical insecticide would result in highest mortality rate.
This experiment applied the One-way Analysis of Variance (ANOVA) to the data to ascertain whether there was a significant difference between the effects of marigold extract as a botanical insecticide in terms of time and dosage. The results showed that there was statistically significant difference under the parameters of time with 10-minute interval observation of 30 minutes and dosage efficacy. These results imply that the null hypothesis should be rejected.
The study suggests extending the observation period for a more comprehensive assessment of its effectiveness on aphids. Additionally, optimizing the formulation by increasing the extract dosage will help determine the optimal amount for maximum efficacy. Lastly, conducting the same experiment on various insect pests will provide valuable insights into the different effects of marigold extract across various pest species.